Phospho-P53 (Ser15) Rekombinanter Antikörper

Phospho-P53 (Ser15) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

2J21

Kat-Nr. : 80195-1-PBS

Synonyme

p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53

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  WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 80195-1-RR (same clone as 80195-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 80195-1-RR (same clone as 80195-1-PBS)

  1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HCT 116 using 80195-1-RR (same clone as 80195-1-PBS)

  Chromatin was prepared from HCT 116 cells treated with Etoposide (50 µM) for 6 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-P53 (Ser15) (80195-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80195-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR Phospho-P53 (Ser15) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)