Phospho-P53 (Ser15) Rekombinanter Antikörper

Phospho-P53 (Ser15) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2J21

Kat-Nr. : 80195-1-RR

Synonyme

p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53

---

• 

  WB analysis using 80195-1-RR

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80195-1-RR

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  IF Staining of HT-29 using 80195-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 80195-1-RR

  1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  ChIP experiment of HCT 116 using 80195-1-RR

  Chromatin was prepared from HCT 116 cells treated with Etoposide (50 µM) for 6 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-P53 (Ser15) (80195-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Etoposid behandelte HT-29-Zellen, mit UV behandelte A431-Zellen
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen
• Erfolgreiche Detektion in FC (Intra): mit UV behandelte A431-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: Etoposide (50 µM, 6 h) HCT 116 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

80195-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Phospho-P53 (Ser15) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

Publikationen

Species	Application	Title
human	WB	Sci Rep; Involvement of JNK signaling in Aspergillus fumigatus-induced inflammatory factors release in bronchial epithelial cells; Authors - Xiao Cui
		Cells; Concurrent Activation of Both Survival-Promoting and Death-Inducing Signaling by Chloroquine in Glioblastoma Stem Cells: Implications for Potential Risks and Benefits of Using Chloroquine as Radiosensitizer; Authors - Andreas Müller
human	WB	Front Pharmacol; Synergistic effects of retinol and retinyl palmitate in alleviating UVB-induced DNA damage and promoting the homologous recombination repair in keratinocytes; Authors - Jiangming Zhong