Phospho-P53 (Ser46) Rekombinanter Antikörper

Phospho-P53 (Ser46) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250307A3

Kat-Nr. : 80077-9-RR

Synonyme

Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46

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  WB analysis using 80077-9-RR

  Non-treated HT-29 cells and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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  WB analysis of A431 using 80077-9-RR

  Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Etoposid behandelte HT-29-Zellen, Mit Nocodazol behandelte A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80077-9-RR bindet in WB, ELISA Phospho-P53 (Ser46) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .