Phospho-PAK1 (Ser144)/PAK2 (Ser141) Rekombinanter Antikörper

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242123D12

Kat-Nr. : 85044-1-PBS

Synonyme

PAK1, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK

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  WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

  Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

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  WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

  Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85044-1-PBS bindet in WB, Indirect ELISA Phospho-PAK1 (Ser144)/PAK2 (Ser141) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Berechnetes Molekulargewicht: 553 aa, 62 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC109299
• Gene symbol: PAK1
• Gene ID (NCBI): 5058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)