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Phospho-PDH E1 Alpha (Ser232) Rekombinanter Antikörper

Phospho-PDH E1 Alpha (Ser232) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1L22

Kat-Nr. : CL488-81491

Synonyme

p PDH E1 Alpha, p PDH E1 Alpha (Ser232), p PDH E1 Alpha Ser232, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated Phospho-PDH E1 Alpha (CL488-81491)

    FC experiment of HEK-293 using CL488-81491

    1x10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Coralite® Plus 488 Phospho-PDH E1 Alpha (Ser232) Recombinant Antibody (CL488-81491, Clone:1L22)(red), or 0.13 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)λ phosphatase treated HEK-293 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-81491 bindet in FC (Intra) Phospho-PDH E1 Alpha (Ser232) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name pyruvate dehydrogenase (lipoamide) alpha 1
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 40 kDa
GenBank-ZugangsnummerBC002406
Gene symbol PDHA1
Gene ID (NCBI) 5160
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)