Phospho-PDH E1 Alpha (Ser293) Rekombinanter Antikörper

Phospho-PDH E1 Alpha (Ser293) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250640D9

Kat-Nr. : 80688-2-PBS

Synonyme

P PDH E1 Alpha (Ser293), Phos PDH E1 Alpha (Ser293), Phos-PDH E1 Alpha (Ser293), Phospho-PDHA1 (Ser293), Phospho-Pyruvate Dehydrogenase α1 (Ser293)

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  WB analysis using 80688-2-RR (same clone as 80688-2-PBS)

  Non-treated HeLa cells, phosphatase inhibitor and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 80688-2-RR (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80688-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80688-2-PBS bindet in WB, Indirect ELISA Phospho-PDH E1 Alpha (Ser293) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC002406
• Gene symbol: PDHA1
• Gene ID (NCBI): 5160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)