Phospho-PDPK1 (Ser241) Rekombinanter Antikörper

Phospho-PDPK1 (Ser241) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250623A11

Kat-Nr. : 85223-3-PBS

Synonyme

PDPK1, 3-phosphoinositide-dependent protein kinase 1, EC:2.7.11.1, hPDK1, PDK1

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  WB analysis of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85223-3-RR (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85223-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85223-3-PBS bindet in WB, Indirect ELISA Phospho-PDPK1 (Ser241) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: 3-phosphoinositide dependent protein kinase-1
• Berechnetes Molekulargewicht: 556 aa, 63 kDa
• Beobachtetes Molekulargewicht: 58-68 kDa
• GenBank-Zugangsnummer: BC012103
• Gene symbol: PDPK1
• Gene ID (NCBI): 5170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.