Phospho-PERK/EIF2AK3 (Ser719) Polyklonaler Antikörper

Phospho-PERK/EIF2AK3 (Ser719) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Rezensionen (1)

Kat-Nr. : 29546-1-AP

Synonyme

p PERK/EIF2AK3, p PERK/EIF2AK3 (Ser719), p PERK/EIF2AK3 Ser719, PERK/EIF2AK3 (Ser719), Phospho PERK/EIF2AK3

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29546-1-AP

  Non-treated HeLa cells and Thapsigargin treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte HeLa-Zellen, Thapsigargin treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 1 publications below

Produktinformation

29546-1-AP bindet in WB, IHC, ELISA Phospho-PERK/EIF2AK3 (Ser719) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: PERK
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publikationen

Species	Application	Title
human	WB	Mar Drugs; Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma; Authors - Yun Huang
human	WB	World J Gastroenterol; Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress; Authors - Xiao-Xue Hou
rat,mouse	WB	Prostaglandins Other Lipid Mediat; Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress; Authors - Long Hao
rat	WB	Pharmaceuticals (Basel); Maltol Improves Peripheral Nerve Function by Inhibiting Schwann Cell Apoptosis via the PERK/eIF2α/CHOP Pathway and MME Upregulation in Diabetic Peripheral Neuropathy; Authors - Jiawei Li
mouse	WB	Ecotoxicol Environ Saf; Endoplasmic reticulum stress promotes oxidative stress, inflammation, and apoptosis: A novel mechanism of citrinin-induced renal injury and dysfunction; Authors - Yongkang Wang
mouse	WB	Am J Physiol Heart Circ Physiol; One day of environment-induced heat stress damages the murine myocardium; Authors - Melissa Roths

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nathalie (Verified Customer) (09-26-2025)

Antibody worked well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116 cells

Phospho-PERK/EIF2AK3 (Ser719) Antibody Western Blot validation (1:1000 dilution) in HCT116 cells (Cat no:29546-1-AP)