Phospho-PERK/EIF2AK3 (Ser719) Monoklonaler Antikörper

Phospho-PERK/EIF2AK3 (Ser719) Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H7A11

Kat-Nr. : 60597-1-PBS

Synonyme

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

---

• 

  WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

  Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60597-1-Ig (same clone as 60597-1-PBS)

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60597-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60597-1-PBS bindet in WB, Indirect ELISA Phospho-PERK/EIF2AK3 (Ser719) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: PERK
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)