Phospho-PERK/EIF2AK3 (Ser719) Rekombinanter Antikörper

Phospho-PERK/EIF2AK3 (Ser719) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243165A4

Kat-Nr. : 81251-2-RR

Synonyme

P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)

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  WB analysis using 81251-2-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81251-2-RR (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Mit λ-Phosphatase behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81251-2-RR bindet in WB, ELISA Phospho-PERK/EIF2AK3 (Ser719) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: PERK
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)