Phospho-PERK/EIF2AK3 (Thr982) Rekombinanter Antikörper

Phospho-PERK/EIF2AK3 (Thr982) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

4E16

Kat-Nr. : 82534-1-RR

Synonyme

p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3

---

• 

  WB analysis of HEK-293 using 82534-1-RR

  Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control.

• 

  WB analysis using 82534-1-RR

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 82534-1-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody (82534-1-RR, Clone:4E16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HEK-293-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:11200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

82534-1-RR bindet in WB, IF, FC (Intra), ELISA Phospho-PERK/EIF2AK3 (Thr982) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: PERK
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
This antibody is equivalent to Thr980 in mice.

Publikationen

Species	Application	Title
human	WB,IF	Int Immunopharmacol; TRx0237 induces apoptosis and enhances anti-PD-1 immunotherapeutic efficacy in anaplastic thyroid Cancer; Authors - Qingyang Ning
		Eur J Pharmacol; Dimethyl Fumarate Improves Non-Alcoholic Fatty Liver Disease by Regulating SIRT1 Signal to Inhibit MAMs Over-Enrichment; Authors - Rui Zhang
human	WB	Sci Rep; LINC00626 drives tamoxifen resistance in breast cancer cells by interaction with UPF1; Authors - Hui Yuan
human	WB	Free Radic Biol Med; Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing; Authors - Zucheng Luo
human	WB	Vet Microbiol; Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replication; Authors - Yu Han
human	WB	Cell Death Dis; Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib; Authors - Yu Feng