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Phospho-PERK/EIF2AK3 (Thr982) Rekombinanter Antikörper

Phospho-PERK/EIF2AK3 (Thr982) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4E16

Kat-Nr. : CL647-82534

Synonyme

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 647-conjugated Phospho-PERK/EIF2AK3 (CL647-82534)

    FC experiment of HEK-293 using CL647-82534

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-PERK/EIF2AK3 (Thr982) Recombinant Antibody (CL647-82534, Clone:4E16)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-82534 bindet in FC (Intra) Phospho-PERK/EIF2AK3 (Thr982) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name eukaryotic translation initiation factor 2-alpha kinase 3
Berechnetes Molekulargewicht 1116 aa, 125 kDa
Beobachtetes Molekulargewicht180 kDa
GenBank-ZugangsnummerBC126354
Gene symbol PERK
Gene ID (NCBI) 9451
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)