Phospho-PKC Alpha (Thr638) Rekombinanter Antikörper

Phospho-PKC Alpha (Thr638) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241844A10

Kat-Nr. : 81575-2-PBS

Synonyme

Phospho-PKC, Phospho-PKC Alpha, Phospho-PKC Alpha Thr638, Phospho-PKC beta (Thr641), Phospho-PKC α

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  WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

  Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 81575-2-RR (same clone as 81575-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated THP-1 cells using Phospho-PKC Alpha (Thr638) antibody (81575-2-RR, Clone: 241844A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 81575-2-RR (same clone as 81575-2-PBS)

  1X10^6 THP-1 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PKC Alpha (Thr638) Recombinant antibody (81575-2-RR, Clone:241844A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81575-2-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Phospho-PKC Alpha (Thr638) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, alpha
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: AK055431
• Gene symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)