Phospho-PKC Alpha (Thr638) Rekombinanter Antikörper

Phospho-PKC Alpha (Thr638) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241844A10

Kat-Nr. : 81575-2-RR

Synonyme

Phospho-PKC, Phospho-PKC Alpha, Phospho-PKC Alpha Thr638, Phospho-PKC beta (Thr641), Phospho-PKC α

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  WB analysis using 81575-2-RR

  Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of THP-1 using 81575-2-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated THP-1 cells using Phospho-PKC Alpha (Thr638) antibody (81575-2-RR, Clone: 241844A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of THP-1 using 81575-2-RR

  1X10^6 THP-1 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PKC Alpha (Thr638) Recombinant antibody (81575-2-RR, Clone:241844A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen
• Erfolgreiche Detektion in FC (Intra): THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81575-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-PKC Alpha (Thr638) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase C, alpha
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: AK055431
• Gene symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)