Phospho-PKN1 (Thr774) Polyklonaler Antikörper

Phospho-PKN1 (Thr774) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29107-1-PBS

Synonyme

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  WB analysis using 29107-1-AP (same clone as 29107-1-PBS)

  Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29107-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29107-1-PBS bindet in WB, Indirect ELISA Phospho-PKN1 (Thr774) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase N1
• Berechnetes Molekulargewicht: 942 aa, 104 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC040061
• Gene symbol: PKN1
• Gene ID (NCBI): 5585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)