• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-PLCG1 (Ser1248) Polyklonaler Antikörper

Phospho-PLCG1 (Ser1248) Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29676-1-PBS

Synonyme

p PLCG1, p PLCG1 (Ser1248), p PLCG1 Ser1248, Phospho PLCG1, Phospho PLCG1 (Ser1248)

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-PLCG1 (Ser1248) Polyclonal antibody (29676-1-AP)

    WB analysis using 29676-1-AP (same clone as 29676-1-PBS)

    Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29676-1-AP (Phospho-PLCG1 (Ser1248) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29676-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-PLCG1 (Ser1248) Polyclonal antibody (29676-1-AP)

    IF Staining of HeLa using 29676-1-AP (same clone as 29676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-PLCG1 (Ser1248) antibody (29676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29676-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29676-1-PBS bindet in WB, IF/ICC, Indirect ELISA Phospho-PLCG1 (Ser1248) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name phospholipase C, gamma 1
Beobachtetes Molekulargewicht145 kDa
GenBank-ZugangsnummerBC144136
Gene symbol PLCG1
Gene ID (NCBI) 5335
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.