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Phospho-POLR2A (Ser7) Rekombinanter Antikörper

Phospho-POLR2A (Ser7) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240829F12

Kat-Nr. : 83932-1-PBS

Synonyme

POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-POLR2A (Ser7) Recombinant antibody (83932-1-RR)

    WB analysis using 83932-1-RR (same clone as 83932-1-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 83932-1-RR (POLR2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83932-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

83932-1-PBS bindet in WB, ELISA Phospho-POLR2A (Ser7) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Berechnetes Molekulargewicht 217 kDa
Beobachtetes Molekulargewicht260 kDa
GenBank-ZugangsnummerNM_000937
Gene symbol POLR2A
Gene ID (NCBI) 5430
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.