Phospho-PRAS40 (Thr246) Rekombinanter Antikörper

Phospho-PRAS40 (Thr246) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250305A2

Kat-Nr. : 80565-2-PBS

Synonyme

p PRAS40 (Thr246), Phospho PRAS40 (Thr246), Phospho-PRAS40, Phospho-PRAS40(Thr246), Phospho-PRAS40-Thr246

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  WB analysis using 80565-2-RR (same clone as 80565-2-PBS)

  Non-treated HeLa cells and Insulin treated HeLa cells were subjected to SDS PAGE followed by western blot with 80565-2-RR (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80565-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80565-2-PBS bindet in WB, Indirect ELISA Phospho-PRAS40 (Thr246) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: AKT1 substrate 1 (proline-rich)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC051844
• Gene symbol: PRAS40
• Gene ID (NCBI): 84335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)