Phospho-PRKD1 (Ser916) Rekombinanter Antikörper

Phospho-PRKD1 (Ser916) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241786A11

Kat-Nr. : 80080-2-PBS

Synonyme

phospho-PKD, Phospho-PKD (Ser916), Phospho-PKD/PKCμ, Phospho-PKD/PKCμ (Ser916), Phospho-PKD/PKCμ Ser916

---

• 

  WB analysis using 80080-2-RR (same clone as 80080-2-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80080-2-RR (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80080-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

80080-2-PBS bindet in WB, Indirect ELISA Phospho-PRKD1 (Ser916) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase D1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: NM_001330069
• Gene symbol: PRKD1
• Gene ID (NCBI): 5587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)