Phospho-Paxillin (Tyr118) Rekombinanter Antikörper

Phospho-Paxillin (Tyr118) Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241063E10

Kat-Nr. : 81180-2-PBS

Synonyme

P Paxillin, P Paxillin (Tyr118), Paxillin (Tyr118), Phospho Paxillin, Phospho Paxillin (Tyr118)

---

• 

  WB analysis using 81180-2-RR (same clone as 81180-2-PBS)

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 81180-2-RR (Phospho-Paxillin (Tyr118) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81180-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 81180-2-RR (same clone as 81180-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-Paxillin (Tyr118) antibody (81180-2-RR, Clone: 241063E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81180-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

81180-2-PBS bindet in WB, IF/ICC, Indirect ELISA Phospho-Paxillin (Tyr118) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: paxillin
• Berechnetes Molekulargewicht: 591 aa, 65 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC136787
• Gene symbol: Paxillin
• Gene ID (NCBI): 5829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)