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Phospho-RASGRF1 (Ser927) Rekombinanter Antikörper

Phospho-RASGRF1 (Ser927) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5K3

Kat-Nr. : 82309-1-PBS

Synonyme

RASGRF1, GRF55, GRF1, GNRP, CDC25L

Filter:
  • Western Blot (WB) analysis of HeLa cells using Phospho-RASGRF1 (Ser927) Recombinant antibody (82309-1-RR)

    WB analysis of HeLa using 82309-1-RR (same clone as 82309-1-PBS)

    Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control. This data was developed using the same antibody clone with 82309-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82309-1-PBS bindet in WB, Indirect ELISA Phospho-RASGRF1 (Ser927) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name Ras protein-specific guanine nucleotide-releasing factor 1
Berechnetes Molekulargewicht 134 kDa
Beobachtetes Molekulargewicht145 kDa
GenBank-ZugangsnummerBC040275
Gene symbol RASGRF1
Gene ID (NCBI) 5923
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)