Phospho-RCC1 (Ser11) Rekombinanter Antikörper

Phospho-RCC1 (Ser11) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230056B10

Kat-Nr. : 83630-1-RR

Synonyme

RCC1, RCC1 I, CHC1, 230056B10

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  WB analysis using 83630-1-RR

  Non-treated and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 83630-1-RR (phospho-RCC1(Ser11) antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) subsequently.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, nocodazole treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83630-1-RR bindet in WB, ELISA Phospho-RCC1 (Ser11) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: regulator of chromosome condensation 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_001048194
• Gene symbol: RCC1
• Gene ID (NCBI): 1104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.