Phospho-RIPK1 (Ser161) Monoklonaler Antikörper

Phospho-RIPK1 (Ser161) Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1B2G1

Kat-Nr. : CL594-66854

Synonyme

RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1

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  IF Staining of HeLa using CL594-66854

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite®594 Phospho-RIPK1 (Ser161) antibody (CL594-66854, Clone: 1B2G1 ) at dilution of 1:200.

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  FC experiment of HEK-293T using CL594-66854

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-RIPK1 (Ser161) (CL594-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): mit Calyculin A behandelte HEK-293T-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66854 bindet in IF/ICC, FC (Intra) Phospho-RIPK1 (Ser161) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 74 kDa, 45 kDa
• GenBank-Zugangsnummer: NM_003804
• Gene symbol: RIPK1
• Gene ID (NCBI): 8737
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25 kDa and 45 kDa in HAEC and HUVEC (PMID: 22685397).