Phospho-S6 Ribosomal protein (Ser235/236) Rekombinanter Antikörper

Phospho-S6 Ribosomal protein (Ser235/236) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241018D12

Kat-Nr. : 80130-2-PBS

Synonyme

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

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  WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

  Non-treated and PDGF (HZ-1215) treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236)) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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  WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

  Non-treated and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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  WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

  Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80130-2-RR (same clone as 80130-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) antibody (80130-2-RR, Clone: 241018D12) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004). This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80130-2-RR (same clone as 80130-2-PBS)

  1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody (80130-2-RR, Clone: 241018D12, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80130-2-PBS bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-S6 Ribosomal protein (Ser235/236) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_001010
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)