Phospho-RPS6KA1 (Ser380) Rekombinanter Antikörper

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• KD/KO Validated

Phospho-RPS6KA1 (Ser380) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

7F23

Kat-Nr. : CL647-80108

Synonyme

RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

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  FC experiment of Jurkat using CL647-80108

  1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RPS6KA1 (Ser380) (CL647-80108, Clone:7F23) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): mit TPA behandelte Jurkat-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80108 bindet in FC (Intra) Phospho-RPS6KA1 (Ser380) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-90 kDa
• GenBank-Zugangsnummer: BC014966
• Gene symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)