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Phospho-RPS6KA1 (Ser380) Rekombinanter Antikörper

Phospho-RPS6KA1 (Ser380) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

7F23

Kat-Nr. : CL647-80108

Synonyme

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 647-conjugated Phospho-RPS6KA1 (Ser (CL647-80108)

    FC experiment of Jurkat using CL647-80108

    1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RPS6KA1 (Ser380) (CL647-80108, Clone:7F23) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCmit TPA behandelte Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80108 bindet in FC (Intra) Phospho-RPS6KA1 (Ser380) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Berechnetes Molekulargewicht 735 aa, 83 kDa
Beobachtetes Molekulargewicht83-90 kDa
GenBank-ZugangsnummerBC014966
Gene symbol RPS6KA1
Gene ID (NCBI) 6195
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)