Phospho-RPS6KA1 (Thr359/Ser363) Monoklonaler Antikörper

Phospho-RPS6KA1 (Thr359/Ser363) Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C11D9

Kat-Nr. : 68041-1-PBS

Synonyme

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

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  WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

  Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control. This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

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  WB analysis using 68041-1-Ig (same clone as 68041-1-PBS)

  Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control. This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68041-1-Ig (same clone as 68041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68041-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68041-1-PBS bindet in WB, IF/ICC, Indirect ELISA Phospho-RPS6KA1 (Thr359/Ser363) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC014966
• Gene symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)