Phospho-RPS6KA1 (Thr359/Ser363) Rekombinanter Antikörper

Phospho-RPS6KA1 (Thr359/Ser363) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3O10

Kat-Nr. : 81475-1-PBS

Synonyme

RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

---

• 

  WB analysis of HeLa using 81475-1-RR (same clone as 81475-1-PBS)

  Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81475-1-RR (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 81475-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 81475-1-RR (same clone as 81475-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-RPS6KA1 (Thr359/Ser363) Recombinant antibody (81475-1-RR, Clone:3O10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81475-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

81475-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-RPS6KA1 (Thr359/Ser363) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-90 kDa
• GenBank-Zugangsnummer: BC014966
• Gene symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)