Phospho-S6 Ribosomal protein (Ser235/236) Polyklonaler Antikörper

Phospho-S6 Ribosomal protein (Ser235/236) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29223-1-PBS

Synonyme

p S6Ribosomal protein, p S6Ribosomal protein (Ser235/236), p S6Ribosomal protein Ser235/236, Phospho RPS6, Phospho RPS6 (Ser235/236)

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  WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

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  WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29223-1-PBS bindet in WB, Indirect ELISA Phospho-S6 Ribosomal protein (Ser235/236) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.