Phospho-S6 Ribosomal protein (Ser235) Monoklonaler Antikörper

Phospho-S6 Ribosomal protein (Ser235) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A4B6

Kat-Nr. : 67898-1-PBS

Synonyme

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

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  WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67898-1-Ig (same clone as 67898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235) antibody (67898-1-Ig, Clone: 2A4B6) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67898-1-Ig (same clone as 67898-1-PBS)

  1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.2 μg Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody (67898-1-Ig, Clone: 2A4B6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67898-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Phospho-S6 Ribosomal protein (Ser235) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.