Phospho-S6 Ribosomal protein (Ser240/244) Rekombinanter Antikörper

Phospho-S6 Ribosomal protein (Ser240/244) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5J13

Kat-Nr. : 81184-1-PBS

Synonyme

p RPS6, p RPS6 (Ser240/244), p S6 Ribosomal protein, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein Ser240/244

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  WB analysis of HEK-293 using 81184-1-RR (same clone as 81184-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (81184-1-RR, Clone: 5J13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81184-1-RR (same clone as 81184-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone:5J13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81184-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Phospho-S6 Ribosomal protein (Ser240/244) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_001010
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.