Phospho-SHP2 (Tyr580) Rekombinanter Antikörper

Phospho-SHP2 (Tyr580) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242434E2

Kat-Nr. : 81219-2-PBS

Synonyme

P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)

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  WB analysis using 81219-2-RR (same clone as 81219-2-PBS)

  Non-treated C6 cells and PDGF treated C6 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81219-2-PBS in a different storage buffer formulation.

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  WB analysis using 81219-2-RR (same clone as 81219-2-PBS)

  Non-treated NIH/3T3 cells and PDGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81219-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81219-2-PBS bindet in WB, Indirect ELISA Phospho-SHP2 (Tyr580) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 11
• Berechnetes Molekulargewicht: 597 aa, 68 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC008692
• Gene symbol: PTPN11
• Gene ID (NCBI): 5781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)