Phospho-SHP2 (Tyr580) Rekombinanter Antikörper

Phospho-SHP2 (Tyr580) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242434E2

Kat-Nr. : 81219-2-RR

Synonyme

P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)

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  WB analysis using 81219-2-RR

  Non-treated C6 cells and PDGF treated C6 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  WB analysis using 81219-2-RR

  Non-treated NIH/3T3 cells and PDGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit PDGF behandelte NIH/3T3-Zellen, PDGF treated C6 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81219-2-RR bindet in WB, ELISA Phospho-SHP2 (Tyr580) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 11
• Berechnetes Molekulargewicht: 597 aa, 68 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC008692
• Gene symbol: PTPN11
• Gene ID (NCBI): 5781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)