Phospho-SMAD1 (Ser187) Polyklonaler Antikörper

Phospho-SMAD1 (Ser187) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28865-1-AP

Synonyme

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser187), SMAD 1, SMAD family member 1, SMAD1

---

• 

  WB analysis using 28865-1-AP

  Non-treated HepG2 and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 28865-1-AP (Phospho-SMAD1 (Ser187) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit BMP2 behandelte HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28865-1-AP bindet in WB, ELISA Phospho-SMAD1 (Ser187) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SMAD family member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS).

Publikationen

Species	Application	Title
rat	WB	Comb Chem High Throughput Screen; Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve; Authors - Wanjing Li
rat	WB	Cardiol Res Pract; Atorvastatin Inhibits Ferroptosis of H9C2 Cells by regulatingSMAD7/Hepcidin Expression to Improve Ischemia-Reperfusion Injury; Authors - You Peng