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Phospho-STAT1 (Tyr701) Rekombinanter Antikörper

Phospho-STAT1 (Tyr701) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C5

Kat-Nr. : CL647-82674-10

Synonyme

p STAT1, p STAT1 (Tyr701), p STAT1 Tyr701, Phospho STAT1, Phospho STAT1 (Tyr701)

Filter:
  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 647-conjugated Phospho-STAT1 (Tyr70 (CL647-82674-10)

    FC experiment of THP-1 using CL647-82674-10

    1X10^6 THP-1 cells untreated (dashed lines) or treated with IFN gamma which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Stat1 (Tyr701) Recombinant Antibody (CL647-82674-10, Clone:1C5)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)IFN gamma treated THP-1 cells

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-82674-10 bindet in FC (Intra) Phospho-STAT1 (Tyr701) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 1, 91kDa
Berechnetes Molekulargewicht 83 kDa
Beobachtetes Molekulargewicht84-91 kDa
GenBank-ZugangsnummerBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).