Phospho-STAT3 (Ser727) Monoklonaler Antikörper
Phospho-STAT3 (Ser727) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
2G11G11
Kat-Nr. : 60479-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, A549-Zellen, MCF-7-Zellen, NIH/3T3-Zellen |
Erfolgreiche Detektion in IF/ICC | UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 2 publications below |
Produktinformation
60479-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-STAT3 (Ser727) und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Ratte |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
Berechnetes Molekulargewicht | 770 aa, 88 kDa |
Beobachtetes Molekulargewicht | 85-90 kDa |
GenBank-Zugangsnummer | BC000627 |
Gene symbol | STAT3 |
Gene ID (NCBI) | 6774 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Protokoll herunterladen |
IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Redox Biol iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy | ||
Stem Cell Res Ther Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway |