Phospho-STAT3 (Ser727) Monoklonaler Antikörper

Phospho-STAT3 (Ser727) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2G11G11

Kat-Nr. : 60479-1-Ig

Synonyme

p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A549-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCUV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below

Produktinformation

60479-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-STAT3 (Ser727) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 3 (acute-phase response factor)
Berechnetes Molekulargewicht 770 aa, 88 kDa
Beobachtetes Molekulargewicht85-90 kDa
GenBank-ZugangsnummerBC000627
Gene symbol STAT3
Gene ID (NCBI) 6774
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgProtokoll herunterladen
IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Redox Biol

iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy

Authors - Hangping Fan
ratWB

Stem Cell Res Ther

Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway

Authors - Zhuyuan Si