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Phospho-STAT5A (Ser726)/STAT5B (Ser731) Rekombinanter Antikörper

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240709D6

Kat-Nr. : CL647-80138-2

Synonyme

p STAT5 (Ser726), p STAT5A, p STAT5A (Ser726), p STAT5A Ser726, Phospho STAT5 (Ser726)

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  • Flow cytometry (FC) experiment of TF-1 cells using CoraLite® Plus 647-conjugated Phospho-STAT5A (Ser7 (CL647-80138-2)

    FC experiment of TF-1 using CL647-80138-2

    1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Stat5a (Ser726)/Stat5b (Ser731) Recombinant Antibody (CL647-80138-2, Clone:240709D6)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit GM-CSF behandelte TF-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80138-2 bindet in FC (Intra) Phospho-STAT5A (Ser726)/STAT5B (Ser731) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 5A
Berechnetes Molekulargewicht 794 aa, 92 kDa
Beobachtetes Molekulargewicht90-95 kDa
GenBank-ZugangsnummerBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)