Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Rekombinanter Antikörper

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2P21

Kat-Nr. : 80115-1-RR

Synonyme

Phospho-STAT5A (Tyr694), STAT5A, STAT5, MGF, 2P21

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  WB analysis using 80115-1-RR

  Non-treated TF-1 and GM-CSF (HZ-1002) treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80115-1-RR (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  FC experiment of TF-1 using 80115-1-RR

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF which intracellularly stained with 0.06 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant antibody (80115-1-RR, Clone:2P21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit GM-CSF behandelte TF-1-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit GM-CSF behandelte TF-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

80115-1-RR bindet in WB, IHC, FC (Intra), ELISA Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 5A
• Berechnetes Molekulargewicht: 794 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC027036
• Gene symbol: STAT5A
• Gene ID (NCBI): 6776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Publikationen

Species	Application	Title
mouse	WB	Carbohydr Polym; Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata; Authors - Wei Xu
	WB	J Immunother Cancer; Endosialin-positive CAFs promote hepatocellular carcinoma progression by suppressing CD8+ T cell infiltration; Authors - Lunbiao Gan
human	WB	Front Immunol; Elevated levels of exogenous prolactin promote inflammation at the maternal-fetal interface via the JAK2/STAT5B signaling axis; Authors - Alycia Williams
mouse	WB	Life Sci Alliance; Identification of TNFAIP2 as a unique cellular regulator of CSF-1 receptor activation; Authors - Randa A Abdelnaser
human	WB	Bioengineered; The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis.; Authors - Guanyang Chen
human,mouse	WB,IHC	Cancer Lett; FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia; Authors - Yuxin Tan