Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoklonaler Antikörper

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F7G5

Kat-Nr. : 68000-1-PBS

Synonyme

STAT5A, STAT5, MGF, 1F7G5

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  WB analysis using 68000-1-Ig (same clone as 68000-1-PBS)

  Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 68000-1-lg (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 68000-1-PBS in a different storage buffer formulation.

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  FC experiment of TF-1 using 68000-1-Ig (same clone as 68000-1-PBS)

  1x10^6 GM-CSF treated TF-1 cells were intracellularly stained with 0.13 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody (68000-1-Ig, Clone:1F7G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.13 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 68000-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68000-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 5A
• Berechnetes Molekulargewicht: 794 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC027036
• Gene symbol: STAT5A
• Gene ID (NCBI): 6776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.