Phospho-STK11/LKB1 (Thr189) Rekombinanter Antikörper

Phospho-STK11/LKB1 (Thr189) Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5J9

Kat-Nr. : CL647-80127

Synonyme

LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1

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  IF Staining of PC-3 using CL647-80127

  Immunofluorescent analysis of (-20°C Ethanol) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using CoraLite® Plus 647 Phospho-STK11/LKB1 (Thr189) antibody (CL647-80127, Clone: 5J9) at dilution of 1:100.

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  FC experiment of PC-3 using CL647-80127

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STK11/LKB1 (Thr189) (CL647-80127, Clone:5J9) , or 0.06 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Mit Calyculin A behandelte PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80127 bindet in IF/ICC, FC (Intra) Phospho-STK11/LKB1 (Thr189) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine/threonine kinase 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC007981
• Gene symbol: LKB1
• Gene ID (NCBI): 6794
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.