Phospho-TAK1 (Ser439) Polyklonaler Antikörper

Phospho-TAK1 (Ser439) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30895-1-AP

Synonyme

p TAK1, p TAK1 (Ser439), p TAK1 Ser439, Phospho TAK1, Phospho TAK1 (Ser439)

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  WB analysis using 30895-1-AP

  Phosphatase inhibitor treated HEK-293T cells, λ phosphatase treated HEK-293T cells, and non-treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 30895-1-AP (Phospho-TAK1 (Ser439) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Mit λ-Phosphatase behandelte HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30895-1-AP bindet in WB, ELISA Phospho-TAK1 (Ser439) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7
• Berechnetes Molekulargewicht: 579 aa, 64 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC017715
• Gene symbol: TAK1
• Gene ID (NCBI): 6885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. TAK1 has been identified as a crucial regulatory component of MAPK and nuclear factor-κB (NF-κB) signaling pathways, which play a key role in lipid metabolism and inflammation. Activated TAK1 then phosphorylates downstream substrates to spark the NF-κB and MAPK signaling pathways. Hence, phospho-TAK1 confers most of its regulatory functions, and inhibiting the hyperactivation of TAK1 should be a promising NASH(Nonalcoholic steatohepatitis ) therapy. (PMID: 34146477) This antibody is equivalent to Ser412 in mice.