Phospho-TAK1 (Thr187) Rekombinanter Antikörper

Phospho-TAK1 (Thr187) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2O6

Kat-Nr. : 81785-1-PBS

Synonyme

p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)

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  WB analysis of HEK-293 using 81785-1-RR (same clone as 81785-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81785-1-RR (same clone as 81785-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81785-1-RR (same clone as 81785-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.13 ug Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR, Clone:2O6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81785-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-TAK1 (Thr187) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7
• Berechnetes Molekulargewicht: 579 aa, 64 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC017715
• Gene symbol: TAK1
• Gene ID (NCBI): 6885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.