Phospho-Tau (Thr181) Polyklonaler Antikörper

Phospho-Tau (Thr181) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 28866-1-AP

Synonyme

MAPT, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, Phospho-TAU (Thr181), pThr181 Tau, TAU

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Galerie der Validierungsdaten

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  WB analysis using 28866-1-AP

  Non-treated SH-SY5Y and PDGF (HZ-1215) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28866-1-AP (Phospho-Tau (Thr181) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit PDGF behandelte SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

28866-1-AP bindet in WB, ELISA Phospho-Tau (Thr181) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: microtubule-associated protein tau
• Berechnetes Molekulargewicht: 37-46, 79-81 kDa
• Beobachtetes Molekulargewicht: 50-80 kDa
• GenBank-Zugangsnummer: BC000558
• Gene symbol: MAPT
• Gene ID (NCBI): 4137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Publikationen

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Basis Dis; Apolipoprotein E ε4 triggers neurotoxicity via cholesterol accumulation, acetylcholine dyshomeostasis, and PKCε mislocalization in cholinergic neuronal cells; Authors - Rebecca Piccarducci
mouse	WB	Neural Plast; Treatment Combining Focused Ultrasound with Gastrodin Alleviates Memory Deficit and Neuropathology in an Alzheimer's Disease-Like Experimental Mouse Model.; Authors - Kaixuan Luo
human	WB	Neurotoxicology; Protective mechanism of gold nanoparticles on human neural stem cells injured by β-amyloid protein through miR-21-5p/SOCS6 pathway; Authors - Guoqing Wang