Phospho-TAU (Thr217) Polyklonaler Antikörper

Phospho-TAU (Thr217) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31138-1-AP

Synonyme

p Tau, p Tau (Thr217), p Tau Thr217, Phospho Tau, Phospho Tau (Thr217)

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  WB analysis using 31138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

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  WB analysis using 31138-1-AP

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: FAD3T mouse brain tissue, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31138-1-AP bindet in WB, ELISA Phospho-TAU (Thr217) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: microtubule-associated protein tau
• Berechnetes Molekulargewicht: 37-46, 79-81 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC000558
• Gene symbol: TAU
• Gene ID (NCBI): 4137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Phosphorylated tau at threonine 217 (p-tau217) is one of the emerging tau markers that has repeatedly shown more promise than other p-tau isoforms as a marker of AD pathology.