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Phospho-TAU (Thr231) Rekombinanter Antikörper

Phospho-TAU (Thr231) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240632B1

Kat-Nr. : CL647-80101-7

Synonyme

p TAU, p TAU (Thr231), p TAU Thr231, Phospho TAU, Phospho TAU (Thr231)

Filter:
  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 647-conjugated Phospho-TAU (Thr231) (CL647-80101-7)

    FC experiment of SH-SY5Y using CL647-80101-7

    1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-TAU (Thr231) Recombinant Antibody (CL647-80101-7, Clone:240632B1)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit λ-Phosphatase behandelte SH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80101-7 bindet in FC (Intra) Phospho-TAU (Thr231) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name microtubule-associated protein tau
Berechnetes Molekulargewicht 37-46, 79-81 kDa
Beobachtetes Molekulargewicht50-80 kDa
GenBank-ZugangsnummerBC000558
Gene symbol TAU
Gene ID (NCBI) 4137
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.