Phospho-TBC1D1 (Ser660) Rekombinanter Antikörper

Phospho-TBC1D1 (Ser660) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

7N19

Kat-Nr. : 81363-2-PBS

Synonyme

TBC1D1, TBC1 domain family member 1, TBC1, KIAA1108, 7N19

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  WB analysis using 81363-2-RR (same clone as 81363-2-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

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  WB analysis using 81363-2-RR (same clone as 81363-2-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81363-2-PBS bindet in WB, Indirect ELISA Phospho-TBC1D1 (Ser660) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC050321
• Gene symbol: TBC1D1
• Gene ID (NCBI): 23216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)