Phospho-TBK1 (Ser172) Rekombinanter Antikörper

Phospho-TBK1 (Ser172) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241787G12

Kat-Nr. : 82382-2-RR

Synonyme

p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172

---

• 

  WB analysis using 82382-2-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82382-2-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

• 

  FC experiment of HeLa using 82382-2-RR

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-TBK1 (Ser172) Recombinant antibody (82382-2-RR, Clone:241787G12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82382-2-RR bindet in WB, FC (Intra), ELISA Phospho-TBK1 (Ser172) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TANK-binding kinase 1
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC034950
• Gene symbol: TBK1
• Gene ID (NCBI): 29110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine/threonine protein kinase TBK1, discovered two decades ago as a member of the IκB kinase (IKK) protein kinase family, plays crucial roles in regulating innate immune response by promoting type I interferon (IFN) and modulating NF-κB signaling. The phosphorylation of Ser172 residue on TBK1 is critical and is commonly used as an indicator for its activity. (PMID: 39279883)