Phospho-TBK1 (Ser172) Rekombinanter Antikörper
Phospho-TBK1 (Ser172) Rekombinant Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
2K2
Kat-Nr. : 82383-1-RR
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mit Calyculin A behandelte HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 7 publications below |
Produktinformation
82383-1-RR bindet in WB, ELISA Phospho-TBK1 (Ser172) und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Rekombinant |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | TANK-binding kinase 1 |
| Beobachtetes Molekulargewicht | 84 kDa |
| GenBank-Zugangsnummer | BC034950 |
| Gene symbol | TBK1 |
| Gene ID (NCBI) | 29110 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Phospho-TBK1 (Ser172) antibody 82383-1-RR | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Virol Pseudorabies virus infection triggers mitophagy to dampen the interferon response and promote viral replication | ||
Cell Rep Non-canonical isoforms of the mRNA polyadenylation factor WDR33 regulate STING-mediated immune responses | ||
Int J Biol Macromol Inhibiting UGCG prevents PRV infection by decreasing lysosome-associated autophage | ||
Br J Pharmacol Glycyrrhetinic acid blocks SARS-CoV-2 infection by activating the cGAS-STING signalling pathway | ||
Eur J Pharmacol Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway | ||
Signal Transduct Target Ther The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marina (Verified Customer) (09-27-2023) | Western blot using p-TBK1 antibody at 1:5000 dilution overnight at 4ºC. Samples are two breast cancer (BC) cell lines, ran in duplicate. Ponceau staining is shown as total protein loading control. A more prominent band can be seen below 100 kDa, and after more exposure, another band at around 80 kDa can be observed.
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