Phospho-TDP43 (Ser409/410) Monoklonaler Antikörper

Phospho-TDP43 (Ser409/410) Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2C1

Kat-Nr. : 66318-1-PBS

Synonyme

p TDP43, p TDP43 (Ser409/410), p TDP43 Ser409/410, Phospho TDP43, Phospho TDP43 (Ser409/410)

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  WB analysis of HeLa using 66318-1-Ig (same clone as 66318-1-PBS)

  Untreated and EA treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66318-1-Ig (same clone as 66318-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66318-1-Ig (same clone as 66318-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66318-1-Ig (same clone as 66318-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410)antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66318-1-Ig (same clone as 66318-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66318-1-PBS bindet in WB, ELISA Phospho-TDP43 (Ser409/410) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa, 35 kDa
• GenBank-Zugangsnummer: NM_007375
• Gene symbol: TDP-43
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.