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Phospho-TDP43 (Ser409/410) Rekombinanter Antikörper

Phospho-TDP43 (Ser409/410) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

6M10

Kat-Nr. : CL647-80007

Synonyme

ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 647-conjugated Phospho-TDP43 (Ser40 (CL647-80007)

    FC experiment of Jurkat using CL647-80007

    1X10^6 Jurkat cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 1 ug CoraLite® Plus 647 Anti-Human Phospho-TDP43 (Ser409/410) (CL647-80007, Clone:6M10) , or 1 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCmit Calyculin A behandelte Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80007 bindet in FC (Intra) Phospho-TDP43 (Ser409/410) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name TAR DNA binding protein
Berechnetes Molekulargewicht 43 kDa
GenBank-ZugangsnummerNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.