Phospho-TSC2 (Ser1387) Rekombinanter Antikörper

Phospho-TSC2 (Ser1387) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242665D1

Kat-Nr. : 80553-2-PBS

Synonyme

TSC2, Tuberin, TSC4, Tuberin/TSC2, tuberous sclerosis 2

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  WB analysis using 80553-2-RR (same clone as 80553-2-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80553-2-RR (Phospho-TSC2 (Ser1387) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody (66305-1-Ig) as the loading control. This data was developed using the same antibody clone with 80553-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80553-2-PBS bindet in WB, Indirect ELISA Phospho-TSC2 (Ser1387) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tuberous sclerosis 2
• Berechnetes Molekulargewicht: 1807 aa, 201 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. AMPK can phosphorylate and activate the Ser1387 site of TSC2.(PMID: 14651849, 12172553, PMID: 23244622)